Large Scale Gene Expression Profiles of Regenerating Inner Ear Sensory Epithelia

摘要: Loss of inner ear sensory hair cells (HC) is a leading cause human hearing loss and balance disorders. Unlike mammals, many lower vertebrates can regenerate these cells. We used cross-species microarrays to examine this process in the avian ear. Specifically, changes expression over 1700 transcription factor (TF) genes were investigated auditory vestibular organs following treatment with two different damaging agents regeneration vitro. Multiple components seven distinct known signaling pathways clearly identifiable: TGFβ, PAX, NOTCH, WNT, NFKappaB, INSULIN/IGF1 AP1. Numerous apoptotic cell cycle control differentially expressed, including p27KIP TFs that regulate its expression. A comparison trends across tissues treatments revealed identical patterns occurred at times during regenerative proliferation. Network analysis gene large dataset also additional presence (and possible network interactions) estrogen receptor signaling, circadian rhythm parts polycomb complex (among others). Equal numbers expressed identified have not yet been placed into any pathway. Specific time points exhibited interesting differences: For example, 45 zinc finger specifically up-regulated later stages cochlear regeneration. These results are first their kind should provide starting point for more detailed investigations role HC recovery, description interactions.