Developmental expression pattern of monoamine oxidases in sensory organs and neural crest derivatives.

摘要: Serotonin (5-HT) has been shown to act as a morphogen in craniofacial and heart development the migration of neural crest derivatives. Some these structures are capable capturing 5-HT during development, but nothing is known about localization main monoamine degradation enzymes, oxidase (MAO) A B, developing tissues. We generated highly specific antibody MAOB; immunoreactivity entirely abolished brain extracts or sections mice lacking MAOB. From use this riboprobes, we report that MAOB expressed early variety derivatives, facial sensory organs, heart. E11.5 P0, was found be strongly following derivatives: aorta, cranial mesenchyme (developing bones, neurons ganglia, cartilages, thyroid, striate muscles), dental mesenchyme, several soft palate boundary cap cells (E11.5-P4). Boundary contribute formation nerve exit-entry points between central peripheral nervous systems. Several organs also contained mRNA, protein, activity. High expression noted olfactory placode, dorsal part epithelium, layer (probably ensheathing glia), cochlear ganglionic cells, taste buds, Merkel vibrissae follicles. Finally, massively pharyngeal organ, heart, liver, mast cells. In contrast, MAOA expresssion restricted sympathetic ganglia meningeal capillary blood vessels. The pattern generally matched previously reported patterns plasma transporter histamine biosynthetic enzyme L-histidine decarboxylase, suggesting role for fine regulation levels embryo. J. Comp. Neurol. 464:392–403, 2003. © 2003 Wiley-Liss, Inc.