Role of integrin α2β1 in mediating osteoblastic differentiation on three-dimensional titanium scaffolds with submicron-scale texture

摘要: Hierarchical surface roughness of titanium and alloy implants plays an important role in osseointegration. In vitro vivo studies show greater osteoblast differentiation bone formation when have submicron-scale textured surfaces. this study, we tested the potential benefit combining a with three-dimensional (3D) structure on involvement integrin-driven mechanism. 3D scaffolds were made using orderly oriented meshes microroughness was added to wire by acid-etching. MG63 human osteoblasts seeded 2D surfaces or without acid etching. At confluence, increased osteocalcin, vascular endothelial growth factor, osteoprotegerin (OPG), alkaline phosphatase (ALP) activity observed comparison at protein level, indicating enhanced differentiation. To further investigate mechanism osteoblast-3D scaffold interaction, integrin α2β1 examined. The results showed β1 silencing abolished increase osteoblastic markers scaffolds. Time course matured faster environment early stage culture, while as cells proliferated, maturation slowed down comparative level After 12 days postconfluent second-phase ALP activity. This study shows that is improved texture mediated α2β1.