Metal complexes of lactoferrin and their effect on the intracellular multiplication of Legionella pneumophila.

作者: Paola Goldoni , Laura Sinibaldi , Piera Valenti , Nicola Orsi

DOI: 10.1023/A:1009221616623

关键词:

摘要: The action of bovine lactoferrin saturated with iron, zinc and manganese on the intracellular multiplication Legionella pneumophila in HeLa cells has been tested. results obtained showed that did not influence invasive efficiency Legionella. bacterium was inhibited by apo-lactoferrin zinc, whereas iron enhanced growth. Experiments parallel were performed citrate to test effect due metal ions alone. Even this condition addition an chelate while produced a certain inhibition.

参考文章(28)
H. A. Shuman, M. A. Horwitz, Legionella pneumophila Invasion of Mononuclear Phagocytes Current Topics in Microbiology and Immunology. ,vol. 209, pp. 99- 112 ,(1996) , 10.1007/978-3-642-85216-9_6
A. P. Dufour, J. M. Barbaree, R. F. Breiman, Legionella: current status and emerging perspectives 4th International Symposium on Legionella, Orlando, Fla. (USA), 1992. ,(1993)
T Furuchi, K Aikawa, H Arai, K Inoue, Bafilomycin A1, a specific inhibitor of vacuolar-type H(+)-ATPase, blocks lysosomal cholesterol trafficking in macrophages. Journal of Biological Chemistry. ,vol. 268, pp. 27345- 27348 ,(1993) , 10.1016/S0021-9258(19)74255-0
T. Yoshimori, A. Yamamoto, Y. Moriyama, M. Futai, Y. Tashiro, Bafilomycin A1, a specific inhibitor of vacuolar-type H(+)-ATPase, inhibits acidification and protein degradation in lysosomes of cultured cells. Journal of Biological Chemistry. ,vol. 266, pp. 17707- 17712 ,(1991) , 10.1016/S0021-9258(19)47429-2
M W Reeves, L Pine, S H Hutner, J R George, W K Harrell, Metal requirements of Legionella pneumophila. Journal of Clinical Microbiology. ,vol. 13, pp. 688- 695 ,(1981) , 10.1128/JCM.13.4.688-695.1981
M W Reeves, L Pine, J B Neilands, A Balows, Absence of siderophore activity in Legionella species grown in iron-deficient media. Journal of Bacteriology. ,vol. 154, pp. 324- 329 ,(1983) , 10.1128/JB.154.1.324-329.1983
P. Valenti, P. Visca, G. Antonini, N. Orsi, E. Antonini, The effect of saturation with Zn2+ and other metal ions on the antibacterial activity of ovotransferrin. Medical Microbiology and Immunology. ,vol. 176, pp. 123- 130 ,(1987) , 10.1007/BF00193893
Carol A. Bortner, Roland R. Arnold, Richard D. Miller, Bactericidal effect of lactoferrin on Legionella pneumophila: effect of the physiological state of the organism. Canadian Journal of Microbiology. ,vol. 35, pp. 1048- 1051 ,(1989) , 10.1139/M89-174
Frederick D. Quinn, Eugene D. Weinberg, Killing of Legionella pneumophila by human serum and iron-binding agents Current Microbiology. ,vol. 17, pp. 111- 116 ,(1988) , 10.1007/BF01568795