Migration distance-based platelet function analysis in a microfluidic system

摘要: Aggregation and adhesion of platelets to the vascular wall are shear-dependent processes that play critical roles in hemostasis thrombosis at injury sites. In this study, we designed a simple rapid assay platelet aggregation microfluidic system. A shearing mechanism using rotating stirrer provided adjustable shear rate time induced activation. When sheared blood was driven through microchannel under vacuum pressure, shear-activated adhered collagen-coated surface, causing flow significantly slow eventually stop. To measure aggregation, migration distance (MD) monitored. As microstirrer speed increased, MD initially decreased exponentially but then increased beyond rpm. For platelet-excluded samples, there were no changes with increasing speed. These findings imply sufficiently high activate is potentially valuable index for measuring shear-dependence Our system quick simple, while providing precise effects on adhesion.