Comparisons of Protein and Peptide Complexity in Poneroid and Formicoid Ant Venoms
作者： Samira R. Aili , Axel Touchard , Jennifer M. S. Koh , Alain Dejean , Jérôme Orivel
DOI: 10.1021/ACS.JPROTEOME.6B00182
关键词:
摘要: Animal venom peptides are currently being developed as novel drugs and bioinsecticides. Because ants use venoms for defense predation, venomous represent an untapped source of potential bioactive toxins. This study compared the protein peptide components poneroid Neoponera commutata, apicalis, Odontomachus hastatus formicoid Ectatomma tuberculatum, brunneum, Myrmecia gulosa. 1D 2D PAGE revealed proteins in mass range 250 kDa. NanoLC-ESI-QTOF MS/MS analysis tryptic presence common also many undescribed proteins. RP-HPLC separation followed by MALDI-TOF MS considerable heterogeneity. It was found that contained between 144 1032 with 5–95% ranges 1–4 1–8 kDa ants, respectively. By employing reducing MALDI matrix 1,5-diaminonapthalene, up to 28 disulfide-bonded pep...
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