Global profiling of proteolytically modified proteins in human metastatic hepatocellular carcinoma cell lines reveals CAPN2 centered network

摘要: Proteolysis affects every protein at some point in its life cycle. Many biomarkers of disease or cancer are stable proteolytic fragments biological fluids. There is great interest and a challenge proteolytically modified study to identify physiologic protease-substrate relationships find potential biomarkers. In this study, two human hepatocellular carcinoma (HCC) cell lines with different metastasis potential, MHCC97L, HCCLM6, were researched high-throughput sensitive PROTOMAP platform. total 391 proteins found be processed many them cleaved into persistent instead completely degraded. Fragments related 161 had expressions these lines. Through analyzing significantly changed bio-informatic tools, several bio-functions such as tumor migration anti-apoptosis enriched. A proteolysis network was also built up, which the CAPN2 centered subnetwork, including SPTBN1, ATP5B, VIM, more active highly metastatic HCC line. Interestingly, modifications CD44 FN1 affect their secretion. This work suggests that plays an important role metastasis.