Construction of a human heart cDNA library and identification of cardiovascular based genes (CVBest)
作者： C. C. Liew , D. M. Hwang , R. X. Wang , S. H. Ng , A. Dempsey
DOI: 10.1007/978-1-4615-6353-2_8
关键词:
摘要: The availability of high quality cDNA libraries is often crucial to the successful identification and characterization genes. concepts potential pitfalls constructing are presented. Various applications requiring outlined, including large-scale single pass sequencing clones generate expressed sequence tags (ESTs) differential screening libraries. usefulness combining such approaches for discovery novel disease-related cardiovascular-based ESTs (CVBest) discussed.
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