Blood mononucleocytes are sensitive to the DNA damaging effects of iron overload--in vitro and ex vivo results with human and rat cells.
作者： Eunju Park , Michael Glei , Yvonne Knöbel , Beatrice L. Pool-Zobel
DOI: 10.1016/J.MRFMMM.2007.01.012
关键词:
摘要: Abstract Iron exposure enhances colorectal carcinogeneis, by producing reactive oxygen species, which damage lipids, proteins and DNA. We recently demonstrated that ferric-nitrilotriacetate (Fe-NTA) damages DNA of human colon cells in different stages malignant transformation. Opposed to this, little is known on systemic effects iron it still difficult determine the border between essential supplementation overload humans. The aim this study was whether Fe-NTA causes global specific peripheral leucocytes. Human leucocytes were treated vitro with for 30 min at 37 °C. Male Sprague Dawley rats fed (6 weeks) an iron-overload diet (9.9 g Fe/kg DM) whole blood collected. measured rat using alkaline version Comet Assay repair enzymes. In distribution TP53 comet images detected fluorescence situ hybridization (Comet FISH) measure region gene. (10–500 μM) clearly genotoxic , also diet. induced approximately two-fold observed previously cells. Oxidized bases vivo while they not . enhanced migration signals into tail leucocytes, indicating a high susceptibility tumour-relevant gene towards overload. conclusion, markedly shows these white are sufficiently sensitive assess iron. measurement could be used as biomarker humans thus results risk.
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