Photoaffinity labels for insect juvenile hormone binding proteins. Synthesis and evaluation in vitro
作者： Glenn D. Prestwich , Ambarish K. Singh , Joan F. Carvalho , John K.K. Oeppe , Gae E. Kovalick
DOI: 10.1016/0040-4020(84)85058-9
关键词:
摘要: Abstract The interactions of insect juvenile hormones (JH) with proteins are critically important to titer regulation, transport, and hormone action at a molecular level. We have synthesized several JH analogs bearing photolabile diazocarbonyl groups as potential photoaffinity labels for binding (JHBP). most promising compound, 10, 11-epoxyfarnesyl diazoacetate ( 2 ) (EFDA) competes III site JHBP from Leucophaea hemolymph ovaries cultured Drosophila cells . Moreover, irradiation protein solutions containing micromolar amounts EFDA gave irreversible loss [ 3 H]-JH capacity no change in affinity the unlabelled protein. could be protected against photoinactivation by presence equimolar during irradiation. High specific activity [10- H]-EFDA was prepared used demonstrate specific, finite receptor Further applications labelling technique characterization cellular localization discussed.
elsevier.com
sci-hub.se 参考文章(48)
Charles S. Hexter, F.H. Westheimer, Intermolecular Reaction during Photolysis of Diazoacetyl α-Chymotrypsin Journal of Biological Chemistry. ,vol. 246, pp. 3928- 3933 ,(1971) , 10.1016/S0021-9258(18)62122-2
K J Kramer, P E Dunn, R C Peterson, H L Seballos, L L Sanburg, J H Law, Purification and characterization of the carrier protein for juvenile hormone from the hemolymph of the tobacco hornworm Manduca sexta Johannson (Lepidoptera: Sphingidae). Journal of Biological Chemistry. ,vol. 251, pp. 4979- 4985 ,(1976) , 10.1016/S0021-9258(17)33210-6
Thomas A. Coudron, Peter E. Dunn, Hidelisa L. Seballos, Robert E. Wharen, Larry L. Sanburg, John H. Law, Preparation of homogeneous juvenile hormone specific esterase from the haemolymph of the tobacco hornworm, Manduca sexta Insect Biochemistry. ,vol. 11, pp. 453- 461 ,(1981) , 10.1016/0020-1790(81)90080-9
K. J. Judy, D. A. Schooley, L. L. Dunham, M. S. Hall, B. J. Bergot, J. B. Siddall, Isolation, Structure, and Absolute Configuration of a New Natural Insect Juvenile Hormone from Manduca sexta Proceedings of the National Academy of Sciences of the United States of America. ,vol. 70, pp. 1509- 1513 ,(1973) , 10.1073/PNAS.70.5.1509
W. Goodman, D. A. Schooley, L. I. Gilbert, Specificity of the juvenile hormone binding protein: The geometrical isomers of juvenile hormone I Proceedings of the National Academy of Sciences of the United States of America. ,vol. 75, pp. 185- 189 ,(1978) , 10.1073/PNAS.75.1.185
Manfred REGITZ, Recent Synthetic Methods in Diazo Chemistry Synthesis. ,vol. 1972, pp. 351- 373 ,(1972) , 10.1055/S-1972-21883
David A. Schooley, B.John Bergot, Walter Goodman, Lawrence I. Gilbert, Synthesis of both optical isomers of insect juvenile hormone III and their affinity for the juvenile hormone-specific binding protein of Manduca sexta. Biochemical and Biophysical Research Communications. ,vol. 81, pp. 743- 749 ,(1978) , 10.1016/0006-291X(78)91414-6
Larry L Sanburg, Karl J Kramer, Ferenc J Kézdy, John H Law, None, Juvenile hormone-specific esterases in the haemolymph of the tobacco hornworm, Manduca sexta Journal of Insect Physiology. ,vol. 21, pp. 873- 887 ,(1975) , 10.1016/0022-1910(75)90015-3
John K. Koeppe, Stephen G. Brantley, Mary M. Nijhout, Structure, haemolymph titre, and regulatory effects of juvenile homone during ovarian maturation in Leucophaea maderae Journal of Insect Physiology. ,vol. 26, pp. 749- 753 ,(1980) , 10.1016/0022-1910(80)90088-8
Thomas C. Sparks, Bruce D. Hammock, Comparative inhibition of the juvenile hormone esterases from Trichoplusia ni, Tenebrio molitor, and Musca domestica Pesticide Biochemistry and Physiology. ,vol. 14, pp. 290- 302 ,(1980) , 10.1016/0048-3575(80)90037-1