Structures of the genes for the β and ε subunits of spinach chloroplast ATPase indicate a dicistronic mRNA and an overlapping translation stop/start signal

摘要: Abstract A 2,4-kilobase-pair region of spinach chloroplast DNA adjacent to the gene for large subunit ribulosebisphosphate carboxylase has been analyzed by RNA hybridization, in vitro transcription/translation, and sequence determination. The analysis indicates that this carries genes β e subunits ATPase two are cotranscribed into a dicistronic mRNA with 4-base-pair overlap between stop codon β-subunit start e-subunit gene. transcribed divergently respect each other. deduced amino acid sequences from show 67% 26% homology, respectively, published Escherichia coli ATPase.