Factors involved in the expression of gene activity in polytene chromosomes.

摘要: In order to separate some of the factors involved in formation puffs antibiotic actinomycin D was applied at different stages puff activity. Puffs were induced by temperature shocks or eodysone. Inhibition RNA synthesis with before application a inducing stimulus prevents neither appearance specific nor accumulation acidic proteins regions. The attained under these conditions approximately 1/3 size normally produced stimulus. Indications obtained that during protein precedes onset synthesis. Synthesis and storage newly synthesized within region studied on basis grain distribution uridine-H3 autoradiographs after various incubation periods. appears be restricted particular area region. After 3 min shock following injection silver grains are located only over formed puff. same becomes labeled 1 pulse stage maximum development. Longer periods result random whole Grain counts areas experimentally regression indicate transferred from where it is stored for certain period Complete release which inhibited maximal activity accomplished 30 35 min.