Trisomy 7 by Dual-Color Fluorescence in Situ Hybridization: A Potential Biological Marker for Prostate Cancer Progression
作者： Judy L. Palmer , Adel K. El-Naggar , Rui Yu Wang , Jan C. Liang , Patricia Troncoso
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摘要: Smear preparations from fine-needle aspirates of 30 prostatic carcinomas obtained radical prostatectomy specimens were examined by a dual-color fluorescence in situ hybridization (FISH) method for the presence chromosome 7 trisomy (chromosome 9 was used as control). The frequency cells with determined tumor and normal epithelial each specimen. Comparison between same patients showed that within all stages, 7/disomy significantly higher than observed (P < 0.0001). Furthermore, mean advanced stages elevated over organ-confined tumors = 0.02). These results are consistent our previous data on paraffin-embedded prostate tissue sections using single-color FISH procedures. However, present study enhances accuracy distinguishing trisomic potentially triploid (trisomy 7/trisomy 9) cells. use rather paraffin provides an easy to examine whole nuclei. Our also suggests better measure genetic instability (e.g., aneuploidy) flow cytometry.
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