Production of ω-hydroxy octanoic acid with Escherichia coli

摘要: Abstract The present proof-of-concept study reports the construction of a whole-cell biocatalyst for de novo production ω-hydroxy octanoic acid. This was achieved by hijacking natural fatty acid cycle and subsequent hydroxylation using specific monooxygenase without need additional feed alkene-like precursors. For this, we used model organism Escherichia coli increased primarily release precursors overexpressing plant thioesterase FatB2 from Cuphea hookeriana in β-oxidation deficient strain, which lead to 2.32 mM (8.38 mg g cww −1 ) 24 h. In order produce corresponding derivative, additionally expressed engineered self-sufficient fusion protein CYP153A Maq (G307A)-CPR BM3 within producing strain. With finally produced 234 μM (0.95 mg g 20 h fed-batch set-up.