Synthesis, depletion and cell-type expression of a protein from the male accessory glands of the dengue vector mosquito Aedes aegypti

摘要: Aedes aegypti males transfer sperm and seminal fluid proteins (Sfps), primarily produced by male accessory glands (AGs), to females during mating. When collectively injected or transplanted into females, AG tissues and/or homogenates have profound effects on female physiology behavior. To identify targets design new strategies for vector control, it is important understand the biology of AGs. Thus, we examined characteristics secretion development in A. aegypti, using AG-specific protein, AAEL010824, as a marker. We showed that AAEL010824 first detectable 12h post-eclosion, increases amount over 3 days adult life. then AAEL0010824 decreases after mating, with each successive mating depleting further; 5 matings no time recovery, its levels are very low. depleted replenished 48 h post-mating. In addition examining level also characterized expression gene. did this making transgenic mosquito line carries an Enhanced Green Fluorescence Protein (EGFP) fused promoter defined here. expressed anterior cells glands, RNA respond further characterizing expression, our results EGFP fusion provide driving expression. By providing information reproductive tissue production one proteins, lay foundation future work aimed at identifying novel population control.