Cytokine detection by antibody-based proximity ligation

作者: M. Gullberg , S. M. Gustafsdottir , E. Schallmeiner , J. Jarvius , M. Bjarnegard

DOI: 10.1073/PNAS.0400552101

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摘要: Among the great challenges in biology are the precise quantification of specific sets of proteins and analyses of their patterns of interaction on a much larger scale than is possible today.This thesis presents a novel protein detection technique-proximity ligation-and reports the development and application of a nucleic acid amplification technique, RCA. Proximity ligation converts information about the presence or co-localization of specific proteins to unique sets of nucleic acid sequences. For detection of target proteins or protein complexes …

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