Immunochemical measurement of histone H3 in non‐nucleosomal compartments of cultured mammalian cells

摘要: We measured histone H3 in the non-nucleosomal compartment of cultured mammalian cells by enzyme-linked immunoelectrotransfer blot assay cytosolic proteins using affinity-purified rabbit anti-H3 IgG, and peroxidase-linked second antibodies. The level was estimated to be 0.5–1.0% nucleosomal content MH-134SC (mean generation time 11 h) 3–4% HeLa 22 h). It showed characteristic changes under inhibition DNA and/or protein synthesis during cell cycle cells. These indicate an inverse relationship between replicating activity nuclear DNA. The possible implication histones regulation gene expression is discussed.