Evaluation of the RealTime HIV-1, Xpert HIV-1, and Aptima HIV-1 Quant Dx Assays in Comparison to the NucliSens EasyQ HIV-1 v2.0 Assay for Quantification of HIV-1 Viral Load

摘要: HIV-1 RNA monitoring, both before and during antiretroviral therapy, is an integral part of HIV management worldwide. Measurements viral loads are expected to assess the copy numbers all common subtypes accurately be equally sensitive at different loads. In this study, we compared for first time performance NucliSens v2.0, RealTime HIV-1, Aptima Quant Dx, Xpert load assays. Plasma samples (n = 404) were selected on basis their v2.0 results subtypes. Concordance, linear regression, Bland-Altman plots assessed, mixed-model analysis was utilized compare analytical assays low high numbers. Overall, concordance (>83.89%), correlation values (Pearson r >0.89), good agreement observed among assays, although which provided most similar outputs (estimated mean 2.67 log copies/ml [95% confidence interval [CI], 2.50 2.84 copies/ml] 2.68 CI, 2.49 2.86 copies/ml], respectively), correlated best with assay (89.8% concordance, Pearson 0.97 0.98). These three exhibited greater precision than assay. All subtype B AG/G 1.60 3.00 copies/ml. The underestimated A1 those >3.00 tended underquantify C (compared assays) samples. efficient detection loads, renders these new suitable clinical laboratories.