Antiviral effects of milk proteins: Acylation results in polyanionic compounds with potent activity against human immunodeficiency virus types 1 and 2 in vitro
作者： P.J. SWART , M.E. KUIPERS , C. SMIT , R. PAUWELS , M.P. DE BÉTHUNE
关键词:
摘要: A number of native and modified milk proteins from bovine or human sources were analyzed for their inhibitory effects on immunodeficiency virus type 1 (HIV-1) HIV-2 in vitro an MT4 cell test system. The investigated lactoferrin, alpha-lactalbumin, beta-lactoglobulin A, B. By acylation the amino function lysine residues proteins, using anhydrides succinic acid cis-aconitic acid, protein derivatives obtained that all showed a strong antiviral activity against and/or 2. IC50 values aconitylated concentration range 0.3 to 3 nM. Succinylation aconitylation alpha-lactalbumin A/B also produced anti-HIV-2 with order 500 3000 All compounds virtually no cytotoxicity at used. Peptide-scanning studies indicated lactoferrin as well charged strongly bind V3 loop gp120 envelope protein, Kd same above-mentioned IC50. Therefore, shielding this domain, resulting inhibition virus-cell fusion entry into cells, may be likely underlying mechanism action.
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D Batinić, F.A. Robey, The V3 region of the envelope glycoprotein of human immunodeficiency virus type 1 binds sulfated polysaccharides and CD4-derived synthetic peptides. Journal of Biological Chemistry. ,vol. 267, pp. 6664- 6671 ,(1992) , 10.1016/S0021-9258(19)50478-1
N. Yahi, J.M. Sabatier, P. Nickel, K. Mabrouk, F. Gonzalez-Scarano, J. Fantini, Suramin inhibits binding of the V3 region of HIV-1 envelope glycoprotein gp120 to galactosylceramide, the receptor for HIV-1 gp120 on human colon epithelial cells. Journal of Biological Chemistry. ,vol. 269, pp. 24349- 24353 ,(1994) , 10.1016/S0021-9258(19)51089-4
Robert W Jansen, Peter Olinga, Geert Harms, Dirk KF Meijer, None, Pharmacokinetic analysis and cellular distribution of the anti-HIV compound succinylated human serum albumin (Suc-HSA) in vivo and in the isolated perfused rat liver. Pharmaceutical Research. ,vol. 10, pp. 1611- 1614 ,(1993) , 10.1023/A:1018972603494
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
FC GREENWOOD, WM HUNTER, JS GLOVER, THE PREPARATION OF 131I-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY Biochemical Journal. ,vol. 89, pp. 114- 123 ,(1963) , 10.1042/BJ0890114
R. L. Jackson, S. J. Busch, A. D. Cardin, Glycosaminoglycans: molecular properties, protein interactions, and role in physiological processes Physiological Reviews. ,vol. 71, pp. 481- 539 ,(1991) , 10.1152/PHYSREV.1991.71.2.481
Elisabeth Mbemba, Vida Chams, Jean-Claude Gluckman, David Klatzmann, Liliane Gattegno, Molecular interaction between HIV-1 major envelope glycoprotein and dextran sulfate. Biochimica et Biophysica Acta. ,vol. 1138, pp. 62- 67 ,(1992) , 10.1016/0925-4439(92)90152-D
Diana Meshcheryakova, Sergei Andreev, Sofia Tarasova, Maria Sidorova, Mariam Vafina, Galina Kornilaeva, Eduard Karamov, Rakhim Khaitov, CD4-derived peptide and sulfated polysaccharides have similar mechanisms of anti-HIV activity based on electrostatic interactions with positively charged gp120 fragments. Molecular Immunology. ,vol. 30, pp. 993- 1001 ,(1993) , 10.1016/0161-5890(93)90124-T
Catherine L. Day, Bryan F. Anderson, John W. Tweedie, Edward N. Baker, Structure of the recombinant N-terminal lobe of human lactoferrin at 2.0 A resolution. Journal of Molecular Biology. ,vol. 232, pp. 1084- 1100 ,(1993) , 10.1006/JMBI.1993.1462
Y. Kamimoto, D. Tanabe, S. Tashiro, T. Hiraoka, Y. Miyauchi, Changes in receptor-mediated endocytosis in liver sinusoidal cells after partial hepatectomy in the rat. Liver. ,vol. 14, pp. 141- 147 ,(2008) , 10.1111/J.1600-0676.1994.TB00063.X