[3H]N-[1-(2-thienyl)cyclohexyl]-3,4-piperidine ([3H]TCP) binding in human frontal cortex: decreases in Alzheimer-type dementia.
作者： Haruaki Ninomiya , Reiko Fukunaga , Takashi Taniguchi , Motohatsu Fujiwara , Shun Shimohama
DOI: 10.1111/J.1471-4159.1990.TB01903.X
关键词:
摘要: : We studied [3H]N-[1-(2-thienyl)cyclohexyl]-3,4-piperidine ([3H]TCP) binding to human frontal cortex obtained at autopsy from 10 histologically normal controls and eight histopathologically verified cases with Alzheimer-type dementia (ATD). Extensively washed membrane preparations were used minimize the effects of endogenous substances. In ATD cortex, total concentration (Bmax) [3H]TCP sites was significantly reduced by 40–50%. The apparent dissociation constant (KD) values showed no significant change. reduction in capacity also Triton X-100–treated preparations, there a linear correlation between number that N-methyl-D-aspartate (NMDA)-sensitive [3H]glutamate sites. spared brains retained affinity for ligand reactivity NMDA, L-glutamate, glycine. These results suggest primary change NMDA receptor-ion channel complex is its number, possibly reflecting loss neurons bearing these receptor complexes, functional linkage within complexes remains normal.
sci-hub.se 参考文章(25)
Graham E. Fagg, Phencyclidine and related drugs bind to the activated N-methyl-D-aspartate receptor-channel complex in rat brain membranes. Neuroscience Letters. ,vol. 76, pp. 221- 227 ,(1987) , 10.1016/0304-3940(87)90719-1
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
Richard W. Ransom, Nancy L. Stec, Cooperative modulation of [3H]MK-801 binding to the N-methyl-D-aspartate receptor-ion channel complex by L-glutamate, glycine, and polyamines. Journal of Neurochemistry. ,vol. 51, pp. 830- 836 ,(1988) , 10.1111/J.1471-4159.1988.TB01818.X
Umberto De Boni, Donald R.Crapper McLachlan, Controlled induction of paired helical filaments of the Alzheimer type in cultured human neurons, by glutamate and aspartate Journal of the Neurological Sciences. ,vol. 68, pp. 105- 118 ,(1985) , 10.1016/0022-510X(85)90093-0
Erik H. F. Wong, Antony R. Knight, Geoffrey N. Woodruff, [3H]MK‐801 Labels a Site on the N‐Methyl‐D‐Aspartate Receptor Channel Complex in Rat Brain Membranes Journal of Neurochemistry. ,vol. 50, pp. 274- 281 ,(1988) , 10.1111/J.1471-4159.1988.TB13260.X
T Wieloch, Hypoglycemia-induced neuronal damage prevented by an N-methyl-D-aspartate antagonist. Science. ,vol. 230, pp. 681- 683 ,(1985) , 10.1126/SCIENCE.2996146
William F. Maragos, Dorothy C.M. Chu, Anne B. Young, Constance J. D'Amato, John B. Penney, Loss of hippocampal [3H]TCP binding in Alzheimer's disease. Neuroscience Letters. ,vol. 74, pp. 371- 376 ,(1987) , 10.1016/0304-3940(87)90326-0
Steven M. Rothman, John W. Olney, Excitotoxity and the NMDA receptor Trends in Neurosciences. ,vol. 10, pp. 299- 302 ,(1987) , 10.1016/0166-2236(87)90177-9
J. Timothy Greenamyre, John B. Penney, Constance J. D'Amato, Anne B. Young, Dementia of the Alzheimer's Type: Changes in Hippocampal L‐[3H]Glutamate Binding Journal of Neurochemistry. ,vol. 48, pp. 543- 551 ,(1987) , 10.1111/J.1471-4159.1987.TB04127.X
Kiyokazu Ogita, Yukio Yoneda, Disclosure by triton X-100 of NMDA-sensitive [3H] glutamate binding sites in brain synaptic membranes. Biochemical and Biophysical Research Communications. ,vol. 153, pp. 510- 517 ,(1988) , 10.1016/S0006-291X(88)81124-0