Comparative study of Hippo pathway genes in cellular conveyor belts of a ctenophore and a cnidarian

摘要: The Hippo pathway regulates growth rate and organ size in fly mouse, notably through control of cell proliferation. Molecular interactions at the heart this are known to have originated unicellular ancestry metazoans. They involve a cascade phosphorylations triggered by kinase Hippo, with subsequent nuclear cytoplasmic shift Yorkie localisation, preventing its binding transcription factor Scalloped, thereby silencing proliferation genes. There few comparative expression data genes non-model animal species none non-bilaterian phyla. All core could be retrieved from ctenophore Pleurobrachia pileus hydrozoan cnidarian Clytia hemisphaerica, important exception ctenophore. Expression study Salvador Scalloped tentacle “cellular conveyor belts” these two organisms revealed striking differences. In P. pileus, their transcripts were detected areas where undifferentiated progenitors intensely proliferate cyclins B D was also seen. C. three expressed not only proliferating but differentiation zone bulb mature cells. However, using an antibody designed against hemiphaerica protein, we show distinct lineages medusa that localisation is predominantly active mainly elsewhere. This first evidence nucleocytoplasmic association arrest cnidarian, strongly evoking division-promoting role protein inhibition activated bilaterian models. Our results furthermore highlight differences terms deployment regulation between cnidarians ctenophores.