Characteristics and EGFP expression of goat mammary gland epithelial cells.

摘要: Contents The aims of this study were (i) to establish a goat mammary gland epithelial (GMGE) cell line, and (ii) determine if these GMGE cells could be maintained long-term in culture by continuous subculturing following transfection with reporter gene, enhanced green fluorescence protein (EGFP). Primary was achieved outgrowth migrating from the fragments tissue lactating goat. The passage 16 transfected EGFP gene using lipofection. expression Cell keratins test immunofluorescence. Βeta-Casein mRNA for RT-PCR. results showed that when grown at low density on plastic substratum, formed islands, confluency, monolayer aggregated characteristic cobble-stone morphology cells. form dome-like structure which looked like nipple, lumen-like structures among Several blister-like appeared appearance contained different types, majority short shuttle-like or polygon beehive-like. A part round flat, small number elongated. Some milk drops. nuclei had 2–4 obvious cores. demonstrated property express transcript encoding protein. successfully transferred into cells, subculturing. In conclusion, we have established (ET-GMGE) line it