Micropropagation of tuna (Opuntia ficus - indica ) and effect of medium composition on proliferation and rooting

摘要: The goal of this study was to determine micropropagation system for a mass production Tuna (Opuntia ficus – indica). For reason, explants dissected from strilled young cladodes successfully established on Murashige and Skoog (MS) medium supplemented with 5 mg l -1 Benzyl amino purine (BAP). MS containing different combinations BAP (5 l-1) Indole acetic acid (IAA) (0, 0.25, 0.5, 1, 2 , Naphtalene (NAA) ( 0, ) (0.5 1 Kinetin 0.5 were tested shoot development . best results elongation obtained in media l-1 0.25 NAA. highest multiplication rate (3.9) observed Satisfactory rooting achieved Basal (5-6 cm length) without callus formation. percentage 100% Plants mix pit perlite (2:1) (100%) acclimatization accomplished under greenhouse condition. In study, total concentration carbohydrates proteins measured vivo cultured (control) vitro propagated tuna after 3 months. As result, no significant differences between control micropropagated protein concentration. Whereas carbohydrate content plants (3.24 g 2- fold that the (1.52 ).