Redistribution of metal ions to control low density lipoprotein oxidation in Ham's F10 medium.
作者： Carole A. Firth , Steven P. Gieseg
DOI: 10.1080/10715760701570099
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摘要: The study of cell-mediated low density lipoprotein (LDL) oxidation has traditionally been undertaken using Ham's F10 media due to its high metal content and levels antioxidants. Although there no acknowledged change this in recent years by the suppliers, medium found be extremely inconsistent promotion LDL absence cells. This variability contrasts with relatively consistent rates THP-1 oxidation. now shown that cell-free is medium-dependent not an artefact experimental protocol. It presents evidence suggests variable are caused iron auto-oxidation during storage medium. can standardized removal all transition metals, treatment Chelex, before addition known amounts or copper. generates a cell culture only allows very slow
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