Cyclic adenosine monophosphate response element-binding protein transcriptionally regulates CHCHD2 associated with the molecular pathogenesis of hepatocellular carcinoma.
作者： RUI SONG , BIAO YANG , XUESONG GAO , JINQIAN ZHANG , LEI SUN
关键词:
摘要: The function of the novel cell migration-promoting factor, coiled-coil-helix-coiled-coil-helix domain containing 2 (CHCHD2) in liver cancer remains to be elucidated. aim present study was elucidate role CHCHD2 carcinogenesis. Immunohistochemistry performed on patients with hepatocellular carcinoma (HCC) and suppression subtractive hybridization (SSH) used for screening differentially expressed genes HepG2 cDNA library. Chronic hepatitis C virus (HCV) infection frequently leads cancer. HCV NS2 protein is a hydrophobic transmembrane that associated certain cellular proteins. Detailed characterization nonstructural (NS2) respect its transregulatory activity lines. A gel electrophoresis mobility shift assay chromatin immunoprecipitation were confirm presence cyclic adenosine monophosphate response element-binding (CREB), transcriptional which specifically interacts promoter. highly HCC specimens consistent tumor markers HCC. identified by SSH cells. upregulated expression monitoring promoter activities. contained 350 bp between nucleotides −257 +93 positively regulated CREB. In conclusion, results indicated may biomarker CREB important activation NS2.
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