MiR-184 Regulates Proliferation in Nucleus Pulposus Cells by Targeting GAS1

摘要: Objective The precise mechanism of nucleus pulposus proliferation in the degeneration intervertebral disk pathogenesis remains to be implicated. MicroRNAs (MiRNAs) are a class 18−22 nucleotides, which small, noncoding RNAs that inhibit protein translation by binding 3′-UTR target gene. Recent studies have shown miRNAs play crucial role various cell biologies such as proliferation, invasion, migration, and cycle. However, miR-184 is still unknown. Method qRT-PCR was performed measure expression miR-184. CCK-8 assay, qRT-PCR, Western blot were used functional (NP) cells. Luciferase assays done find Result We demonstrated upregulated degenerative NP tissues compared with control tissues, positively correlated grade. identified Growth Arrest Specific Gene 1 ( GAS1 ) direct gene cells, ectopic promoted cells proliferation. In addition, we found downregulated inversely grade degeneration. Moreover, overexpression could induce AKT phosphorylation decreased overexpressing Conclusion These results /Akt pathway may potential therapeutic for disc