Developmental Expression of Calcium-Binding Protein-Containing Neurons in Neocortical Transplants

摘要: Abstract The present study examined the development of calcium binding protein-containing neurons in a timed series fetal neocortical transplants. immunoexpression parvalbumin and calbindin, which are subpopulations GABAergic neurons, have been widely studied normal disease injury states. Because their purported resistance to oxidative by ability buffer Ca++ influx, these particularly following ischemia. it is likely that stress associated with grafting procedure, we sought determine if displayed enhanced survival characteristics. Normally, calbindin represent about 5–10% cortical neurons. Within 2–4 wk after expression both proteins increased markedly relatively larger number (27% for parvalbumin) were immunopositive. This increase was transitory, however, 4 mo beyond, confocal microscopic data showed reduction over 50% (+) processes. Calbindin processes qualitative change they smaller less terminal branching. Electron microscopy confirmed substantial synaptic contacts. Interestingly, older grafts, remaining those strongly NSE suggesting link between metabolism buffering grafted It possible early grafts certain neuronal populations transiently upregulated as defensive mechanism against influx stress. Over time, physiological normalization within protein diminished, possibly due lack appropriate afferent input interneuronal pool.