A novel fluorescent aptasensor based on silica nanoparticles, PicoGreen and exonuclease III as a signal amplification method for ultrasensitive detection of myoglobin
作者： Khalil Abnous , Noor Mohammad Danesh , Ahmad Sarreshtehdar Emrani , Mohammad Ramezani , Seyed Mohammad Taghdisi
DOI: 10.1016/J.ACA.2016.02.036
关键词:
摘要: Measurement of myoglobin (Mb) in human blood serum is great interest for quick diagnosis acute myocardial infarction (AMI). In this study, a novel fluorescent aptasensor was designed ultrasensitive and selective detection Mb, based on target-induced high fluorescence intensity, complementary strand aptamer (CS), PicoGreen (PG) dye, exonuclease III (Exo III) silica nanoparticles coated with streptavidin (SNPs-Streptavidin). The developed obtains characteristics SNPs as enhancers Exo an enzyme which selectively digests the 3'-end double-stranded DNA (dsDNA), PG dye could bind to dsDNA selectivity sensitivity (Apt) toward its target. absence no free CS remains environment SNPs-Streptavidin, resulting weak emission. present dsDNA-modified SNPs-Streptavidin complex forms, leading very strong exhibited specificity Mb limit (LOD) low 52 pM. addition, efficiently used detect serum.
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