A fluorescent aptasensor for amplified label-free detection of adenosine triphosphate based on core–shell Ag@SiO2 nanoparticles
作者： Quanwei Song , Manshu Peng , Le Wang , Dacheng He , Jin Ouyang
DOI: 10.1016/J.BIOS.2015.09.008
关键词:
摘要: The novel, facile and universal aptamer-based methods for the highly sensitive and selective fluorescence detection of important biomolecules have attracted considerable interest. Here, we present a label-free aptasensor for adenosine triphosphate (ATP) detection in aqueous solutions by using an ultra-sensitive nucleic acid stain PicoGreen (PG) as a fluorescent indicator and core–shell Ag@ SiO 2 nanoparticles (NPs) as a metal-enhanced fluorescence (MEF) platform. In the presence of ATP, the complementary DNA …
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