Aptamer switch probe based on intramolecular displacement.

摘要: A novel aptamer-based molecular probe design employing intramolecular signal transduction is demonstrated. The composed of three elements: an aptamer, a short, partially cDNA sequence, and PEG linker conjugating the aptamer with short DNA strand. We have termed this "aptamer switch probe", or ASP. ASP utilizes both fluorophore quencher which are respectively modified at two termini In absence target molecule, will hybridize keeping in close proximity, thus switching off fluorescence. However, when meets its target, binding between molecule disturb hybridization, move away from fluorophore, and, effect, on Both ATP human alpha-thrombin aptamers were engineered to demonstrate design, showed that fluorescence enhancement could be quantitatively mediated by addition various amounts molecules. these ASPs presented excellent selectivity prompt response toward their targets. With intrinsic advantages resulting architecture, holds promising potential for future applications, such as biochip situ imaging, require reusability, stability, response, high sensitivity.