Comparison of transient protein expression in tobacco leaves and plant suspension culture.

摘要: Transient gene expression is being developed to provide a more rapid means of assessing plant tissues as protein production platform without the labor-intensive and time-consuming process generating stably transformed transgenic plants. gus-intron reporter was facilitated in three different tobacco species. Two approaches T-DNA delivery were compared: (1) infiltration prototrophic strain Agrobacterium into leaves (2) coculture cell suspension cultures with an auxotroph. Wounding wire brush prior had large positive impact on Nicotiana benthamiana but not for tabacum or glutinosa. The best level achieved by leaf N. (0.025% total soluble protein). A culture line glutinosa greater than 0.04% TSP. tissue culture-based technique therefore provides improved levels transient under aseptic conditions facilitate improvements control environments.