The gene for rat atrial natriuretic factor.

摘要: Atrial natriuretic factor (ANF), a peptide hormone recently isolated from heart atria, appears to play an important role in the regulation of extracellular fluid volume and blood pressure. Indeed, natural synthetic ANF rapidly markedly stimulate natriuresis diuresis produce smooth muscle relaxation. Consistent with hypothesis that is novel hormone, it was shown present circulation, high affinity membrane receptors specific for have been described renal, vascular, adrenal tissues. These biological activities suggest conditions like hypertension could be associated defective gene expression. We others by cDNA cloning part larger precursor, pro-natriodilatin (PND). now describe isolation structural analysis rat PND gene. Southern blot genomic DNA suggests presence single per haploid genome. The coding sequences are interrupted two short introns. A long alternating purine-pyrimidine tract (GT)9GATG(GT)27 found 111 base pairs downstream polyadenylation site; such adopt Z-DNA configuration they appear very active intergenic recombination. Comparison human shows highest homology 5'-flanking as well sequences. will useful model study physiology pathology this regulator cardiovascular system.