Detection of tilapia metallothionein using antibody-immobilized quartz crystal microbalance sensor
作者： Namsoo Kim , So-Hee Son , Woo-Yeon Kim
DOI: 10.1016/J.SNB.2014.03.034
关键词:
摘要: Abstract Metallothionein (MT) was induced in tilapia by three subcutaneous injections of CdCl2 and the recovered MT protein purified to homogeneity through DEAE-cellulose ion exchange chromatography Sephacryl S-100 gel filtration. For use as biological component, a polyclonal anti-tilapia antibody prepared using MT. During preparation sensor, topological changes pertinent immobilization thiolated antibody, subsequent blocking surface with BSA final immune reaction were observed AFM imaging. Specificity sensor confirmed comparison responses defective intact chips, chips that immobilized BSA. Concentration-dependent showed linear relationship Y (log10 response) = 0.3438X MT) + 0.7368 (r = 0.9606) double-logarithmic plot limit detection 20 ng/mL. When serum from compared control serum, induction detectable even at 20,000-fold dilution serum.
sci-hub.se 参考文章(54)
Woo-Yeon Kim, Hyung-Seok Ryu, Namsoo Kim, Flatfish vitellogenin detection using optical waveguide lightmode spectroscopy-based immunosensor. Journal of Microbiology and Biotechnology. ,vol. 17, pp. 1445- 1451 ,(2007)
Eckschlager T, Kizek R, Fabrik I, Hrabeta J, Krizkova S, Adam, Metallothionein--a promising tool for cancer diagnostics. Bratislavské lekárske listy. ,vol. 110, pp. 93- 97 ,(2009)
In-Seon Park, Namsoo Kim, Thiolated Salmonella antibody immobilization onto the gold surface of piezoelectric quartz crystal1This paper was presented at the fifth World Congress on Biosensors, Berlin, Germany, 3–5 June 1998.1 Biosensors and Bioelectronics. ,vol. 13, pp. 1091- 1097 ,(1998) , 10.1016/S0956-5663(98)00067-0
James P. Fabisiak, Gregory G. Borisenko, Shang-Xi Liu, Vladimir A. Tyurin, Bruce R. Pitt, Valerian E. Kagan, Redox sensor function of metallothioneins Methods in Enzymology. ,vol. 353, pp. 268- 281 ,(2002) , 10.1016/S0076-6879(02)53055-X
K.M. Chan, PCR-cloning of goldfish and tilapia metallothionein complementary DNAs. Biochemical and Biophysical Research Communications. ,vol. 205, pp. 368- 374 ,(1994) , 10.1006/BBRC.1994.2674
Kristina Van Houdt, Inge Nicasi, Els Van Mechelen, Bea Veulemans, Marc De Ley, Monoclonal antibodies against metallothioneins from the human liver Immunology Letters. ,vol. 32, pp. 21- 26 ,(1992) , 10.1016/0165-2478(92)90193-R
Mayra L. González-Félix, Francisco J. Castillo-Yañez, Víctor M. Ocaño-Higuera, Martin Perez-Velazquez, Venecia Cota-Moreno, José Lozano-Taylor, Effect of dietary protein source and time on alkaline proteolytic activity of Nile tilapia (Oreochromis niloticus). Fish Physiology and Biochemistry. ,vol. 36, pp. 779- 785 ,(2010) , 10.1007/S10695-009-9352-4
Aldo Viarengo, Enrica Ponzano, Francesco Dondero, Rita Fabbri, A simple spectrophotometric method for metallothionein evaluation in marine organisms: an application to Mediterranean and Antarctic molluscs Marine Environmental Research. ,vol. 44, pp. 69- 84 ,(1997) , 10.1016/S0141-1136(96)00103-1
Jamel Jebali, Mohamed Banni, Hamadi Gerbej, Hamadi Boussetta, Juan López-Barea, José Alhama, Metallothionein induction by Cu, Cd and Hg in Dicentrarchus labrax liver: assessment by RP-HPLC with fluorescence detection and spectrophotometry. Marine Environmental Research. ,vol. 65, pp. 358- 363 ,(2008) , 10.1016/J.MARENVRES.2008.01.002
Kyung Wook Wee, Ghi Yuun Kang, Jaebum Park, Ji Yoon Kang, Dae Sung Yoon, Jung Ho Park, Tae Song Kim, Novel electrical detection of label-free disease marker proteins using piezoresistive self-sensing micro-cantilevers Biosensors and Bioelectronics. ,vol. 20, pp. 1932- 1938 ,(2005) , 10.1016/J.BIOS.2004.09.023