After Four Hours of Cold Ischemia and Cardioplegic Protocol, the Heart Can Still Be Rescued With Postconditioning

摘要: BACKGROUND: There is evidence that ischemia lasting more than 4 hours affects cardiac allograft survival. Ischemia and reperfusion are associated with additional deleterious effects. Protective effects of preconditioning already being used but protocols based on postconditioning have not been evaluated. We tested the impact hearts maintained in cold for a long period total global we compared results those obtained pyruvate, cardioprotective molecule. METHODS: Isolated working rat were subjected to (4 h/4 degrees C), followed by 45 min reperfusion. Postconditioning consisted brief applied three times during onset (ischemia: 30 sec, reperfusion: sec). Superoxide anion production collagen content evaluated cryosections. RESULTS: Our showed led improvements functions comparable conferred pyruvate. reduced myocardial damage, gave better functional recovery, preserved content. It duration arrhythmias at In group, this improvement was reduction superoxide production. CONCLUSIONS: conclusion, our study induced good hr/4 C) protocol lower O2 part mediated NAPDH oxidase activity. interesting note that, experimental conditions, beneficial produced