Development of Multilocus Sequence Typing (MLST) for Mycoplasma synoviae
作者： Mohamed El-Gazzar , Mostafa Ghanem , Kristina McDonald , Naola Ferguson-Noel , Ziv Raviv
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摘要: SUMMARY Mycoplasma synoviae (MS) is a poultry pathogen that has had an increasing incidence and economic impact over the past few years. Strain identification necessary for outbreak investigation, infection source identification, facilitating prevention control as well eradication efforts. Currently, segment of variable lipoprotein hemagglutinin A (vlhA) gene (420 bp) only target used MS strain identification. major limitation this assay colonality typed samples can be inferred if their vlhA sequences are identical; however, different, degree relatedness uncertain. In study we propose multilocus sequence typing (MLST) to further refine After initial screening 24 housekeeping genes potential targets, seven were selected MLST assay. An internal (450–711 from each was successfully amplified sequenced 58 different str...
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