Expression of functional IL 2 receptors by lipopolysaccharide and interferon-gamma stimulated human monocytes.

摘要: Human peripheral blood monocytes were stimulated with lipopolysaccharide (LPS) and interferon-gamma (IFN-gamma) alone or in combination. Stimulated but not resting displayed the Tac peptide of interleukin 2 (IL 2) receptor within 24 hr as measured by immunofluorescence staining [3H] binding. The total number anti-Tac binding sites on co-stimulated was 13,700. By using scatchard analysis radiolabeled IL 2, activated cells shown to express low numbers (below 100 sites/cell) high affinity a KD approximately 15 pM. LPS IFN-gamma additive augmenting sites. an ELISA assay specific for soluble released form we identified 112 U/ml receptors supernatant IFN-gamma, 233 after stimulation LPS, 519 addition both stimulating agents. Both membrane (55,000 daltons), well (45,000 50,000 daltons) Tac, receptor, from immunoprecipitation gel electrophoresis be similar size comparable forms these derived T cells. In addition, 8 contained mRNA specifically hybridizing cDNA probe coding peptide. Finally, responded recombinant increase H2O2 production that fluorescent indicator 2,7-dichlorofluorescein. This response observed induction monocytic may point functional role this during monocyte/macrophage responses microbial infections.