Stimulation of mucosal T cells in situ with anti-CD3 antibody: location of the activated T cells and their distribution within the mucosal micro-environment.

摘要: Mucosal T cells in explants of human fetal small intestine (17-20 weeks gestation) organ culture were activated situ using monoclonal anti-CD3 antibody. Changes the distribution within mucosa, and their phenotype, monitored by immunohistochemistry on frozen sections. Anti-CD3 stimulated (as determined expression CD25) predominantly lamina propria rarely seen epithelium. In control cultures, after 72 h, CD3+ IEL decreased to low numbers compared day zero. However, cultures treated with anti-CD3, maintained some experiments significantly increased zero levels. At onset 50-60% CD4-, 8-, virtually all HML-1+. The cell infiltrate into epithelium induced activation was also mostly CD3+, 4-, These provide strong evidence that increases can be a consequence activation.