MicroRNA-184 inhibits cell proliferation and invasion, and specifically targets TNFAIP2 in Glioma.

摘要: miRNA-184 is an oncogene in human hepatocellular carcinoma but acts as a tumor suppressor tongue squamous cell carcinoma. Studies have shown that miR-184 was down-regulated glioma and TNFα-induced protein 2 (TNFAIP2) closely related to tumorigenesis. This study aimed determine the functions of mechanisms miRNA-184-TNFAIP2 mediated progression. Real-time reverse-transcription PCR detected expression TNFAIP2. U87 U251 cells were transfected with mimic, inhibitor, or negative control miRNA, their invasion abilities assayed. Cellular proliferation measured by counting kit-8 assay. effects on apoptosis cycle assessed flow cytometer. Biological information software predicted could target (TNFAIP2), Which further validated Western blot qRT-PCR cells. In vivo, transduced either lentiviral over-expressed lentivirus injected into nude mice subcutaneously intracranial respectively. Expression significantly lower tissues cell-lines compared normal brain tissues. Protein mRNA TNFAIP2 inversely correlated glioma. vitro, also decreased after transfection mimic. xenografted size overexpressing group smaller than miR-NC group. Concordantly, mimic had higher rate, triggering accumulation at G0/G1 phase S-phase. regulate affected its translation inhibit progression might serve novel therapeutic