Towards a competitive solid state fermentation: Cellulases production from coffee husk by sequential batch operation and role of microbial diversity.

摘要: The cost of cellulases is the main bottleneck for bioethanol production at commercial scale. Solid-state fermentation (SSF) a promising technology that can potentially reduce by using wastes as substrates. In this work, SSF system 4.5 L bioreactors was operated continuously sequential batch operation fermented solids from one to inoculate following batch. Coffee husk used lignocellulosic substrate. Compost starter in first provide rich microbiota. Two strategies were applied: 10% inoculum 48 h batches (SB90) and 50% 24 (SB50). A consistent robust process achieved operation. Similar cellulase activities around 10 Filter Paper Units per gram dry obtained through both strategies. Microbial diversity starting materials final characterized next generation sequencing. composition similar but relative abundance families species affected strategy used. Main bacteria came compost (families Sphingobacteriaceae, Paenibacillaceae Xanthomonadaceae), while fungi coffee Phaffomycetaceae, Dipodascaceae two unidentified class Tramellomycetes). There high presence non-identified mycobiota solids. identified Pseudoxanthonomas taiwanensis (12.3% SB50 6.1% SB90) Sphingobacterium composti (6.1% 2.6% yeasts Cyberlindnera jardinii Barnettozyma californica (17.8 4.1% respectively 34 9.1% SB90), all four previously described lignocellulose degraders. development these operational further biological characterization end product could eventually benefit economics providing standard specialized continuous production.