Psychromonas antarcticus gen. nov., sp. nov., A new aerotolerant anaerobic, halophilic psychrophile isolated from pond sediment of the McMurdo ice shelf, antarctica

摘要: A gram-negative, rod- to oval-shaped, aerotolerant anaerobic bacterium was isolated from an enrichment inoculated with sediment taken below the cyanobacterial mat of a high-salinity pond near Bratina Island on McMurdo Ice Shelf, Antarctica. The organism positive for terminal oxidase and catalase motile by means polar flagellum. Optimal growth cultures occurred at 12° C, pH 6.5, NaCl concentration 3% (w/v). Of variety polysaccharides tested, only starch glycogen supported growth. No observed cellulosic substrates xylan, unable attack esculin. Monosaccharides disaccharides, including cell-wall constituent N-acetyl glucosamine, were fermented. Per 100 mol hexose, following products (in mol) formed: acetate, 60; formate, 130; ethanol, 56; lactate, 73; CO2, 15; butyrate, 2. Propionate, n-propanol, n-butanol succinate not detectable in culture medium (< 1 per monomer). Hydrogen detected head space (detection limit < 10–5 atm). Growth yields aerobic static liquid slightly higher than those culture, fermentation favoured acetate expense electron sink products. inhibited shaking cultures, did utilize nitrate or sulfate as acceptors. G+C content DNA 42.8 mol%. phylogenetic analysis indicated that is member γ-subgroup Proteobacteria, but it distinct other members this group based sequence its 16S rRNA gene, mol% G+C, morphology, physiological biochemical characteristics. It designated new genus species; type strain star-1 (DSM 10704).