Enzyme-assisted optimized supercritical fluid extraction to improve Medicago sativa polyphenolics isolation
作者： Aneta Krakowska , Katarzyna Rafińska , Justyna Walczak , Bogusław Buszewski
DOI: 10.1016/J.INDCROP.2018.08.004
关键词:
摘要: Abstract The aim of this study was to develop an effective Medicago sativa extraction method using enzyme-assisted supercritical fluid (EA-SFE). response surface methodology (RSM) based on Box-Behnken design chosen optimize the parameters process, i.e., temperature, pressure and percentage co-solvent for total phenolics content antioxidants. Commercial enzyme preparation kemzyme applied M. leaves. antioxidant in optimal conditions from enzymatically hydrolyzed leaves carried out by (SFE) with 96% ethanol as a co-solvent. Optimal TPC activity were: temperature 68 °C, 205 bar 15.5% addition. For sake comparison, conventional performed. obtained results showed that use commercial formulation allowed phenolic compounds extract increase compared control. HPLC-MS/MS characterization revealed digested material much more efficient (142.69 ± 5.11 μg/g) than non-digested (94.17 ± 4.80 μg/g) or (57.42 ± 2.25 μg/g). Our work offers protocol enhanced release biological active plant cells.
sci-hub.se 参考文章(38)
Sofía Cavero, Mónica R. García-Risco, Francisco R. Marín, Laura Jaime, Susana Santoyo, F. Javier Señoráns, Guillermo Reglero, Elena Ibañez, Supercritical fluid extraction of antioxidant compounds from oregano: Chemical and functional characterization via LC–MS and in vitro assays Journal of Supercritical Fluids. ,vol. 38, pp. 62- 69 ,(2006) , 10.1016/J.SUPFLU.2005.01.003
Zoran Zeković, Jaroslava Švarc-Gajić, Aleksandra Cvetanović, Marija Radojković, Branimir Pavlić, Optimization of the Polyphenolics Extraction from Chamomile Ligulate Flowers Using Response Surface Methodology International Journal of Plant Research. ,vol. 4, pp. 43- 50 ,(2014)
Muhammad Mushtaq, Bushra Sultana, Farooq Anwar, Ahmad Adnan, Syed SH Rizvi, None, Enzyme-assisted supercritical fluid extraction of phenolic antioxidants from pomegranate peel Journal of Supercritical Fluids. ,vol. 104, pp. 122- 131 ,(2015) , 10.1016/J.SUPFLU.2015.05.020
M.S. Liza, R. Abdul Rahman, B. Mandana, S. Jinap, A. Rahmat, I.S.M. Zaidul, A. Hamid, Supercritical carbon dioxide extraction of bioactive flavonoid from Strobilanthes crispus (Pecah Kaca) Food and Bioproducts Processing. ,vol. 88, pp. 319- 326 ,(2010) , 10.1016/J.FBP.2009.02.001
Vernon L. Singleton, Rudolf Orthofer, Rosa M. Lamuela-Raventós, ANALYSIS OF TOTAL PHENOLS AND OTHER OXIDATION SUBSTRATES AND ANTIOXIDANTS BY MEANS OF FOLIN-CIOCALTEU REAGENT Methods in Enzymology. ,vol. 299, pp. 152- 178 ,(1999) , 10.1016/S0076-6879(99)99017-1
Chang-Liang Jing, Xiao-Fang Dong, Jian-Ming Tong, Optimization of Ultrasonic-Assisted Extraction of Flavonoid Compounds and Antioxidants from Alfalfa Using Response Surface Method Molecules. ,vol. 20, pp. 15550- 15571 ,(2015) , 10.3390/MOLECULES200915550
Anna Stochmal, Sonia Piacente, Cosimo Pizza, Francesco De Riccardis, Rick Leitz, Wieslaw Oleszek, None, Alfalfa (Medicago sativa L.) Flavonoids. 1. Apigenin and Luteolin Glycosides from Aerial Parts Journal of Agricultural and Food Chemistry. ,vol. 49, pp. 753- 758 ,(2001) , 10.1021/JF000876P
Shuo Chen, Xin-Hui Xing, Jian-Jun Huang, Ming-Shu Xu, Enzyme-assisted extraction of flavonoids from Ginkgo biloba leaves: improvement effect of flavonol transglycosylation catalyzed by Penicillium decumbens cellulase. Enzyme and Microbial Technology. ,vol. 48, pp. 100- 105 ,(2011) , 10.1016/J.ENZMICTEC.2010.09.017
Wieslaw Oleszek, Anna Stochmal, Ana M. Simonet, Francisco A. Macias, Alfalfa (Medicago sativa L.) flavonoids. 2. Tricin and chrysoeriol glycosides from aerial parts Journal of Agricultural and Food Chemistry. ,vol. 49, pp. 5310- 5314 ,(2001) , 10.1021/JF010600X
Sónia A.O. Santos, Juan J. Villaverde, Carlos M. Silva, Carlos P. Neto, Armando J.D. Silvestre, Supercritical fluid extraction of phenolic compounds from Eucalyptus globulus Labill bark The Journal of Supercritical Fluids. ,vol. 71, pp. 71- 79 ,(2012) , 10.1016/J.SUPFLU.2012.07.004