SIRT1/3 Activation by Resveratrol Attenuates Acute Kidney Injury in a Septic Rat Model

摘要: Sepsis often results in damage to multiple organ systems, possibly due severe mitochondrial dysfunction. Two members of the sirtuin family, SIRT1 and SIRT3, have been implicated reversal damage. The aim this study was determine role SIRT1/3 acute kidney injury (AKI) following sepsis a septic rat model. After drug pretreatment cecal ligation puncture (CLP) model reproduction rats, we performed survival time evaluation tissue extraction renal tubular epithelial cell (RTEC) isolation. We observed reduced activity, elevated acetylated SOD2 (ac-SOD2) levels oxidative stress, damaged mitochondria RTECs sepsis. Treatment with resveratrol (RSV), chemical activator, effectively restored levels, ameliorated stress function RTECs, prolonged time. However, beneficial effects RSV were greatly abrogated by Ex527, selective inhibitor SIRT1. These suggest therapeutic for AKI rat, which may rely on SIRT3-mediated deacetylation SOD2. activation could therefore be promising strategy treat sepsis-associated AKI.