In vivo and in vitro effect of adoptive immunotherapy of experimental murine brain tumors using lymphokine-activated killer cells.

摘要: Adoptive immunotherapy for the experimental murine brain tumor was investigated by using lymphokine-activated killer (LAK) cells both in vitro and vivo. Supernatants of 48-h culture medium spleen from Wistar rats presence concanavalin A were used as interleukin 2 (IL-2). LAK generated cocultivation Fischer with IL-2 peak reactivity on Day or 3 culture. Lytic activity observed against not only syngenic but also allogenic xenogenic cells, while no lytic normal cells. The cell depletion test, dye exclusion immunofluorescence method monoclonal antibodies revealed that partially belonged to population activated T-cell group, precursor did react any used. On basis these results vivo study performed. immune adoptively transferred i.v. intratumorally (i.t.) seventh day after inoculation T9, a gliosarcoma induced methylcholanthrene rats, into right basal ganglia. Then survival rate necrotic foci compared between groups treated those control. significantly higher than control (administered i.v.; P less 0.01, administered i.t.; 0.05). But treatment effective. incidence area tumors greater others. Microautoradiography performed [3H]thymidine-labeled which models 14th T9. It accumulated lung shortly administration then liver spleen, especially white pulp. inhibitor sera tumor-bearing (P 0.001), it depressed markedly cyclophosphamide 0.01). adoptive transfer may be one effective treatments malignant tumor. nature inhibitors is necessary clarified more immunotherapy.