Viral myocarditis and coagulopathy: increased tissue factor expression and plasma thrombogenicity.

摘要: We investigated the effects of viral infection on Tissue Factor (TF) expression and activity in mice within myocardium to understand increased thrombosis during myocarditis. Mice were infected with coxsackie virus B3 (CVB3) hearts collected at day 4, 8 28 post (p.i.). Myocardial TF cellular as well plasma analyzed from CVB3 by Western blot, chromogenic Xa generation assay, situ staining for active immunohistochemistry. In addition expression, hemodynamic parameters measured time course infection. Furthermore, we myocardial tissues patients suspected inflammatory cardiomyopathy. protein was maximally 5-fold elevated days p.i. remained (P<0.001 vs. non-infected controls). Alterations associated fibrin deposits myocardium. The pathway inhibitor not altered Active co-localized CD3 positive cells VCAM-1 endothelial positively correlated amount infiltrating Mac3 (Spearman-Rho rho=0.749 P<0.0001 CD3(+) rho=0.775 Mac3(+); N=35). Increased a 2-fold (P<0.05 human hearts, an cell activation marker (rho=0.523 CD62E; N=54). Viral myocarditis is hypercoagulative state which activity. Upregulation contributes systemic coagulation cascade.