Microwave Accelerated Green Synthesis of Stable Silver Nanoparticles with Eucalyptus globulus Leaf Extract and Their Antibacterial and Antibiofilm Activity on Clinical Isolates

摘要: A simple and rapid microwave assisted method of green synthesis silver nanoparticles (AgNPs) was developed using aqueous leaf extract Eucalyptus globulus(ELE), their antibacterial antibiofilm potential investigated. With this aim, the solutions ELE AgNO3(1 mM) were mixed (1:4 v/v), irradiated at 2450 Mhz, for 30 sec. The instant color change ELE-AgNO3 mixture from pale yellow to dark brown indicated ELE-AgNPs synthesis. intensity peak 428 nm in UV-Vis spectra, due surface plasmon resonance AgNPs, varied with amount ELE, AgNO3 concentration, pH time incubation. biosynthesized characterized by UV-visible spectroscopy, XRD, TEM, SEM-EDX, FTIR TGA analyses. size determined be range 1.9–4.3 5-25 nm, without treatment, respectively. SEM exhibited capping AgNPs constituents, validated analysis. data revealed presence plant organic constituents metabolites bound ELE-AgNPs, which contributes stability. antimicrobial activity assessed growth biofilm inhibition extended spectrum β-lactamase (ESBL) producing Pseudomonas aeruginosa, Escherichia coli methicillin-resistant Staphylococcus aureus (MRSA) methicillin-sensitive (MSSA) clinical bacterial isolates. results demonstrated that S. more sensitive than E. P. aeruginosa. MRSA higher MSSA, whereas aeruginosa treatment. Also, significant (83 ± 3% 84 5%) observed case elucidated environmentally friendly, economical quick production colloidal bio-functionalized effectual applications, as broad agents inhibitors.