Green synthesis of Al2O3 nanoparticles and their bactericidal potential against clinical isolates of multi-drug resistant Pseudomonas aeruginosa.

摘要: The high prevalence of extended-spectrum β-lactamases (76.3 %) and metallo-β-lactamases (7.3 %) amongst the bacteria Pseudomonas aeruginosa is a critical problem that has set forth an enormous therapeutic challenge. suggested role nanoparticles as next generation antibiotics, inadequate information on antibacterial activity aluminium oxide led us to investigate green synthesis (Al2O3 NPs) using leaf extracts lemongrass its against clinical isolates P. aeruginosa. synthesized Al2O3-NPs were characterized by scanning electron microcopy, resolution-transmission microscopy, atomic force X-ray diffraction, Zeta potential, differential light scattering techniques. diffraction data revealed average size spherical 34.5 nm. hydrodynamic in Milli Q water potential determined be 254 nm +52.2 mV, respectively. minimal inhibitory concentration was found range 1,600-3,200 µg/ml. Treatment at concentrations >2,000 µg/ml, resulted complete growth inhibition isolates. Scanning microcopy analysis clusters attached bacterial cell surface, causing structural deformities treated cells. High microscopy confirmed crossed membrane become intracellular. interaction with eventually triggered loss integrity, most likely due intracellular oxidative stress. explicitly can exploited effective bactericidal agent β-lactamases, non-extended-spectrum strains aeruginosa, regardless their drug resistance patterns mechanisms. results elucidated significance developing regimen multi-drug resistant infections. use extract for appears cost effective, nontoxic, eco-friendly strong offers compatibility pharmaceutical other biomedical applications.