Rat Studies Comparing 11C-FMAU, 18F-FLT, and 76Br-BFU as Proliferation Markers

摘要: We analyzed and compared 1-(2′-deoxy-2′-fluoro-β-d-arabinofuranosyl)-[methyl-11C]thymine (11C-FMAU), 3′-deoxy-3′-[18F]fluorothymidine (18F-FLT) 1-(2′-deoxy-2′-fluoro-β-d-arabinofuranosyl)-5-[76Br]bromouracil (76Br-BFU) with respect to tissue uptake, DNA incorporation, excretion modulation in rats. The goal of the investigation was evaluate efficiency 3 nucleoside tracers as potential for measuring proliferation. Methods: Sprague-Dawley rats were divided into groups administered 5 MBq 11C-FMAU, 1 18F-FLT, or 2 76Br-BFU. For each tracer, a subgroup also 6 mg/kg cimetidine. 11C-FMAU group killed at 5, 20, 40, 60, 80 min after injection; 18F-FLT 4 h; 76Br-BFU 2, 4, 6, 24 h. Samples blood, liver, kidney, spleen, intestine taken, radioactivity measured. separation made samples fraction Results: Maximal uptake seen spleen intestine, organs active synthesis. highest relative 60 h group. In group, increased gradually during observation period, markedly receiving Cimetidine did not affect groups. 78% 80% 97% DNA-incorporation only 2% Conclusion:76Br-BFU predominantly incorporates has great PET tracer assessment proliferation vivo. may have marker, but time is limited. does incorporate therefore direct marker