Differential sensitivity of A549 non-small lung carcinoma cell responses to epidermal growth factor receptor pathway inhibitors.

摘要: It has been demonstrated that A549 non-small cell lung cancer (NSCLC) cells are sensitive to epidermal growth factor receptor (EGFR) inhibitors in vivo xenograft animal models, but relatively resistant conventional vitro monolayer assays. Here, we utilized anchorage-independent growth/survival assays as well motility and these tests detect the effects of two EGFR inhibitors, small molecule inhibitor AG1478 ligand-blocking antibody 225 mAb, on more sensitively than was effective mAb at inhibiting EGF-stimulated growth, part due its pronounced ability inhibit survival, whereas were both able motility. In order determine which signalling pathway components most strongly associated with responses, analyzed parallel phosphorylation levels itself several downstream elements. We found limited MAPK, PI3K STAT3 correlated inability promote anchorage independent apoptosis, did not correlate Based our results cells, propose EGF stimulates tumour progression NSCLC largely through