Ascorbic acid reversibly inhibits proliferation of retinal pigment epithelial cells.

摘要: . Purpose: Proliferation control in adult retinal pigment epithelial (ARPE) cells is an essential factor the clinical management of proliferative vitreoretinopathy (PVR). Factors which inhibit PVR and are without toxic potential therefore interest controlling proliferation. The aim present study was to gain insight into a possible function high intraocular ascorbic acid levels as physiological modulator proliferation. Methods: Adult were incubated vitro with increasing concentrations (0.5–4 mmol, pH 7.4). Cell proliferation assayed by bromide-deoxy-uridine (BrdU) assay. culture medium (CM) containing replaced normal CM recovery measured after 24 hours. In order be able distinguish between inhibition, apoptosis, necrosis proliferation, we performed TUNEL assays fluorescence analysis cell-counter (FAC) analysis. Results: Ascorbic significantly inhibits ARPE cell if it above 2 mmol. Proliferation resumed all cultures pre-incubation acid, indicating that direct toxicity negligible factor. time-point extent dependent on initial concentration. Fluorescence-labelled counts apoptosis markers data showed some induction incubation 4 mmol acid. Conclusions: Ascorbic has dose-dependent influence vital cells. This possibly reflects role at level within vitreous cavity preventing These findings may stimulate development new strategies treatment PVR.