Development of a specific fragment pattern-based quadrupole-Orbitrap mass spectrometry method to screen adulterated products of phosphodiesterase-5 inhibitors and their analogues

摘要: Abstract Recently, adulterated supplements with phosphodiesterase-5 inhibitors (PDE-5i) have frequently observed. New synthetic analogues obtained from the chemical modification of parent compounds are found in illicit products despite continuous efforts to inspect for these adulterants. A rapid and accurate method based on quadrupole-Orbitrap mass spectrometry was developed simultaneously confirming quantifying 85 PDE-5i derived present erectile dysfunction (ED). Common ions according their similar structures were proposed MS/MS fragmentations. These common could be an important diagnosis presence targets or new emerging supplements. Several validation parameters employed, resulting a limit detection quantification 0.09–8.55 ng/mL 0.24–17.10 ng/mL, respectively. The linear correlation coefficient (r2) higher than 0.995, mean recoveries target range 82–118%. total 187 products, on/offline markets over period 3 years (2015–2017), screened by established method. Approximately 53% them at concentrations 0.1–726.0 mg/g products. In interests public health, this study describes determine